US pop star Justin Timberlake has kicked off the Australian leg of his world tour at Brisbane's Boondall Entertainment Centre by making a $100,000 donation to Australia Zoo.

The multi-award winning artist stopped the show on Saturday night to tell his screaming fans he had taken a private tour of the Sunshine Coast attraction during his last visit to the country and had been very impressed.

"And when I came here last time, it really hit me... I said to myself 'what can I do?'

"So what I did was, 50 cents from every ticket sold on the Australian tour goes to Wildlife Warriors."

The star then presented zoo director Wes Mannion with a cheque for $100,000.